Tubulin binding protein, CacyBP/SIP, induces actin polymerization and may link actin and tubulin cytoskeletons

Treadmilling of tubulin and actin.

Chaperonin-mediated folding of actin and tubulin

T HE central dogma of molecular biology holds that proteins contain within their primary amino acid sequence all the information that is required to dictate their three-dimensional structure. In principle, therefore, proteins can fold spontaneously (2). It is now clear, however, that the proper folding of many proteins requires facilitation. The facilitation reaction is accomplished via interac...

Identifying the dynamics of actin and tubulin polymerization in iPSCs and in iPSC-derived neurons

The development of the nervous system requires cytoskeleton-mediated processes coordinating self-renewal, migration, and differentiation of neurons. It is not surprising that many neurodevelopmental problems and neurodegenerative disorders are caused by deficiencies in cytoskeleton-related genes. For this reason, we focus on the cytoskeletal dynamics in proliferating iPSCs and in iPSC-derived n...

Bacterial Actin and Tubulin Homologs in Cell Growth and Division

In contrast to the elaborate cytoskeletal machines harbored by eukaryotic cells, such as mitotic spindles, cytoskeletal structures detectable by typical negative stain electron microscopy are generally absent from bacterial cells. As a result, for decades it was thought that bacteria lacked cytoskeletal machines. Revolutions in genomics and fluorescence microscopy have confirmed the existence n...

Tubulin and actin topology during zygote formation of Saccharomyces cerevisiae.

The topology of tubulin and actin during mating of Saccharomyces cerevisiae was analysed by fluorescence microscopy with the monoclonal anti-tubulin antibody Tu01 and rhodamine-labelled phalloidin. Preconjugatory cells displayed an asymmetric distribution of the microtubule and actin cytoskeleton and an overall polarization of the cells preceding cell fusion. Prior to karyogamy, the haploid spi...